Developing regulatory biochemical reaction circuits
Abstract
Statement of objectives: The proposed research will apply the organizational power of DNA nanotechnology to the challenges of engineering biochemical reaction circuits with regulatory and feedback functions. The specific aims are: (1) Design and construct aptamer lock-controlled DNA nanotweezers that switch conformations upon binding to target pathway molecules. (2) Use designed DNA nanotweezers to assemble multienzyme pathways that can be regulated through feedback of product generation or substrate consumption. (3)Develop logic-gated swinging arms to channel the transfer of intermediates in multienzyme pathways for controlling pathway activity and specificity in response to molecular inputs. (4) Construct an artificial electron transport chain to facilitate electron transfer along a redox potential gradient within the reaction pathway.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 08, 2019
- Accession Number
- AD1070662
Entities
People
- Jinglin Fu
Organizations
- Rutgers University