A 3D Bioprinted Model for the Study of Premalignant Breast Disease

Abstract

This proposal involves a multidisciplinary team including a surgical oncologist, a mammary gland biologist, a biomedical engineer, and a cancer biologist. We hypothesize that a novel in vitro 3D bioprinted model of premalignant breast cells growing within a breast ductal system will represent the first and most faithful representation of premalignant progression in vitro and will be an outstanding model for identify markers of low-risk premalignant disease which doesnt require treatment. In the yr 1 of the proposal we quantified mammary gland development, finding strain dependent differences highlighting a genetic component to development, and in yr 2 we continued to characterize development. In year 1 we developed a proof-of-principle 3D printing technology to print a mammary gland, and in year 2 we comprehensively developed a 3D printed ductal system with perfusion to study breast cells in their 3D environment. In yr 3 (NCE) we will examine growth and behavior of breast cells in this 3D mammary duct in vitro.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2018
Accession Number
AD1070935

Entities

People

  • Adrian V Lee
  • Elizabeth Catherine Firestone

Organizations

  • University of Pittsburgh

Tags

DTIC Thesaurus Topics

  • Additive Manufacturing
  • Cells
  • Curvature
  • Demographic Cohorts
  • Diameters
  • Diffusion
  • Dihedral Angle
  • Engineers
  • Environment
  • Geometry
  • Glands
  • Mammary Glands
  • Measurement
  • Medical Personnel
  • Perfusion
  • Printing
  • Three Dimensional

Fields of Study

  • Biology

Readers

  • Manufacturing Engineering.
  • Molecular Biology and Genetics
  • Women's Health and Cancer Risk Research: African American Women and Pregnancy Outcomes.

Technology Areas

  • Biotechnology