Optimization of RNA Body FluID Assays: Differential Co-Extraction for Sperm Analysis and Rapid Direct Lysis for High-Throughput Evidence Screening

Abstract

The aims of the current work were to custom-develop for DFSC casework use: (i) a differential DNA/RNA co-extraction isolation protocol for the separation and analysis of non-sperm and sperm fractions in sexual assault evidence (i.e. prior to DNA profiling and identification of body fluid of origin); and (ii) a rapid RNA direct lysis method for higher throughput screening of forensic biological evidence (blood, semen, saliva, vaginal secretions and menstrual blood).

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Document Details

Document Type
Technical Report
Publication Date
Aug 09, 2018
Accession Number
AD1071869

Entities

People

  • Jack Ballantyne

Organizations

  • University of Central Florida

Tags

Communities of Interest

  • Air Platforms

DTIC Thesaurus Topics

  • Body Fluids
  • Capillary Electrophoresis
  • Data Analysis
  • Data Sets
  • Electronic Mail
  • Factor Analysis
  • Gene Expression
  • High Temperature
  • Identification
  • Identification Systems
  • Law
  • Materials
  • Mrna
  • Polymerase Chain Reaction
  • Sexual Assault
  • Standards
  • Websites

Fields of Study

  • Chemistry

Readers

  • Immunology
  • Oncology and Biomarker-Based Cancer Detection.