Evaluating an Upper Respiratory Disease Panel on the Portable MinION Sequencer

Abstract

The MinION nanopore sequencer was released to community testers for evaluation using a variety of sequencing applications. The MinION was used to evaluate upper respiratory disease infections and was found to have tremendous potential for field use. In this study, we tested the ability of the MinION to accurately identify and differentiate clinical bacterial and viral samples via targeted sequencing and whole genome sequencing. The current nanopore technology has limitations with respect to error rate but has steadily improved with development of new flow cells and library kits.

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Document Details

Document Type
Technical Report
Publication Date
Apr 24, 2019
Accession Number
AD1072114

Entities

People

  • Brian Geier
  • Clarise R. Starr
  • James Baldwin
  • Wanda J. Lyon
  • Zachary K. Smith

Organizations

  • 711th Human Performance Wing

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Air Force
  • Air Force Research Laboratories
  • Chemistry
  • Culture Techniques
  • Data Analysis
  • Detection
  • Diseases And Disorders
  • Genetic Structures
  • Health Services
  • Infection
  • Infectious Diseases
  • Mers-Cov
  • Microbiology
  • Microbiomes
  • Sars
  • Viruses
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Geospatial Intelligence and Artificial Intelligence Analytics
  • Immunology and Pathology
  • Molecular Genetics