The Effects of Endovascular Perfusion Augmentation for Critical Care (EPACC) in a Porcine (Sus scrofa) Model of Sepsis
Abstract
Objectives: To evaluate the effects of endovascular aortic occlusion in a porcine model of sepsis. Methods: Animals were anesthetized and instrumented. Sepsis was induced by an intravenous infusion of live Pseudomonas aeruginosa titrated to obtain a mean arterial pressure less than 65 mmHg while avoiding pulmonary hypertension. When the target pressure was achieved some animals received endovascular aortic occlusion. Animals were resuscitated with isotonic crystalloids and vasopressors according to a prespecified algorithm. Results: We observed a large variability in cardiovascular responses to the bacterial infusion with some animals suffering profound hypotension non-responsive to resuscitation efforts while others remained stable with minimal support. Endovascular aortic occlusion yielded variable responses as well. Conclusion: This sepsis model has cardiovascular effects that are variable between animals. Data acquired from the experiment will be used for future sepsis-related fluid responsiveness studies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 16, 2019
- Accession Number
- AD1072198
Entities
People
- Guillaume L. Hoareau
Organizations
- David Grant USAF Medical Center