Development of Field Methodology to Rapidly Detect Dehalococcoides and Dehalobacter Spp. Genes On-Site

Abstract

Nucleic acid amplification of biomarkers is increasingly used to measure microbial activity and predict remedial performance in sites with trichloroethene (TCE) contamination. Field-based genetic quantification of microorganisms associated with bioremediation may help increase accuracy that is diminished through transport and processing of groundwater samples. Sterivex(trademark) cartridges and a previously undescribed mechanism for eluting biomass was used to concentrate cells. DNA extraction-free loop mediated isothermal amplification (LAMP) was monitored in real-time with a point of use device (termed Gene-Z).

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2019
Accession Number
AD1073415

Entities

People

  • Alison M Cupples
  • Paul B. Hatzinger
  • Robert D. Stedtfeld
  • Syed A. Hashsham

Organizations

  • Michigan State University

Tags

DTIC Thesaurus Topics

  • Accuracy
  • Alcohols
  • Alkanes
  • Alkenes
  • Bacterial Infections
  • Bacteriology
  • Biodegradation
  • Birds
  • Chemical Reactions
  • Chemical Synthesis
  • Chemistry
  • Department Of Defense
  • Digital Data
  • Ecology
  • Environmental Health
  • Environmental Protection
  • Laboratory Equipment
  • Microbial Genome
  • Microbiology
  • Microbiomes
  • Microorganisms
  • Organic Chemistry
  • Spreadsheet Software

Fields of Study

  • Environmental science

Readers

  • Analytical Chemistry
  • Groundwater Contamination Remediation.
  • Oncology

Technology Areas

  • Biotechnology
  • Biotechnology - Bioremediation