Methods for Inactivation of Venezuelan Equine Encephalitis Virus
Abstract
We used Venezuelan equine encephalitis (VEE) virus strain TC-83 as a model to study the effects of chemical, thermal, and irradiation conditions on infectivity of single-stranded positive RNA viruses. Our data showed that exposure of the virus to 65 degrees C for 5-15 min resulted in a 5-6 log reduction of virus infectivity. Treatment with 100-500 mM NaOH caused at least a 4 log reduction in infectivity. Treatment with Beta-propiolactone at 50-200 mM concentrations for 15-60 min caused complete or near-complete loss of virus infectivity. Treatment with TRIzol LS reagent at a 3:1 (v/v) TRIzol reagent/virus suspension (1 x 10^7 TCID_50/mL, where TCID_50 is the tissue-culture infective dose that will result in 50 percent culture infection) induced complete loss of infectivity. Effects of increasing doses of cobalt-60 irradiation (1-40 kGy) showed a linear inverse relationship between irradiation dose and virus infectivity as measured by TCID_50 assay. Irradiation doses of >20 kGy caused >4 log reduction in infectivity. At the 40 kGy dose, complete loss of infectivity occurred when a virus titer was 1 x 10^7 TCID_50/mL. From these results, we estimate the 1 log reduction dose and sterility assurance level to be approximately 5.5 and 32.9 kGy, respectively.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2019
- Accession Number
- AD1074319
Entities
People
- Amber Prugh
- Daniel Angelini
- Jacquelyn Harris
- M. Sofi Ibrahim
- Michelle Ziemski
- Todd Sickler
- Tracey Biggs
Organizations
- United States Army Soldier Systems Center