Determination of Fluorescence Emission and UV-Vis-NIR Absorbance for Nanomaterials Solution Using a HORIBA Scientific NanoLog (Registered trademark) Spectrofluorometer

Abstract

A fluorescence spectroscopy technique has been widely used for characterizing the fluorescence of nanomaterials. The technique provides fast and sensitive measurements that are applicable in many research and industrial areas. Obtained absorbance, excitation, and fluorescence emission spectra can be used to determine sample concentrations, binding kinetics of biomolecules, reaction mechanisms, and many more applications. This SOP provides general guidance for using fluorescence spectroscopy techniques with an instrument such as a HORIBA Scientific NanoLog(registered trademark) spectrofluorometer to determine fluorescence emission and UV-Vis-NIR absorbance of nanomaterials in solution. Procedures and recommendations of sample preparation, instrument preparation, sample analysis, and results analysis are briefly described. The procedure was tested on a variety of nanomaterial solutions.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2019
Accession Number
AD1074355

Entities

People

  • Alan James Kennedy
  • Kathryn Kremer
  • Qihua Wu
  • Stephen Gibbons

Organizations

  • Engineer Research and Development Center

Tags

Communities of Interest

  • Biomedical
  • Energy and Power Technologies
  • Sensors

DTIC Thesaurus Topics

  • Chemistry
  • Detection
  • Detectors
  • Electromagnetic Radiation
  • Emission Spectra
  • Ground State
  • Light Sources
  • Materials
  • Measurement
  • Nanomaterials
  • Nanoparticles
  • Nanotechnology
  • Quantum Dots
  • Quantum Yields
  • Spectra
  • Spectroscopy
  • Test Methods

Fields of Study

  • Chemistry

Readers

  • Nanoscale Plasmonic Nanotechnology