Acute Dermal Toxicity Study of Aircraft Engine Oils Using New Zealand White Rabbits (Oryctolagus cuniculus)

Abstract

There is little data available on toxicity levels of used aircraft engine oils relative to their unused (new) versions. This study was conducted to determine if new engine oils and their used versions have the potential to induce irritation. Twelve Male New Zealand White Rabbits (Oryctolaguscuniculus, 19 weeks old) were used to determine the acute dermal toxicity potential of four aircraft engine oils in their unused and used states: two MIL-PRF-7808 oils, a MIL-PRF-23699 HTS oil and an experimental MIL-PRF-23699 type oil. Five fur-free test sites (6 cm2 each) located lateral to the midline of the back were treated with two undiluted (0.5 ml) new engine oils and their used versions. The fifth site received RO water as a control. Each treatment was repeated 3 times (3 rabbits/oil type). Each oil was tested under both semi-occluded and occluded conditions. E-collars were placed on each animal for at least 72 h to prevent ingestion of the test substance and/or gauze plus wrappings and disturbance of site recovery. The 4 hour exposure was followed by gauze plus wrappings removal, and gently cleaning of sites prior to scoring for erythema and edema at 0.5-1, 24, 48 and 72 h post exposure based on Draize (1959). Additional observations were made on days 7, 10 and 14 to determine recovery. Exposure to both used and new oils produced dermal irritation consisting of no more than very slight to well-defined erythema and very slight edema. Calculated Primary Dermal Irritation Index (PDII) indicate that all the oils were slightly irritating. Although the PDII values for new oils and their used versions were not significantly different, they were all statistically higher (p less than 0.05) than those obtained for the control regardless the type of occlusion binding applied.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2018
Accession Number
AD1074887

Entities

People

  • David R. Mattie
  • Isaie Sibomana
  • Lewis Rosado
  • Neal A. Good
  • Patrick T. Hellman

Organizations

  • 711th Human Performance Wing

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Air Force
  • Air Force Research Laboratories
  • Aircraft Engine Oils
  • Aircraft Engines
  • Aircrafts
  • Bandages
  • Biological Sciences
  • Environmental Protection
  • Epidermis
  • Health Services
  • Laboratory Animals
  • Medical Personnel
  • New Zealand
  • Skin Diseases
  • Statistical Analysis
  • Toxicity
  • Word Processors

Readers

  • Petroleum Engineering
  • Toxicology/Environmental Toxicology
  • Trauma Surgery or Emergency Medicine.