Optimization and Application of a Small Laccase (SLAC) for Enzymatic Fuel Cell Development
Abstract
This report describes technical effort in the development of enzymatic fuel cells for power generation, specifically in optimization of a 'small laccase' (SLAC) from Streptomyces coelicolor. The work includes an optimized method for purification of homogeneous SLAC by recombinant expression in Escherichia coli. The improved expression and purification method provides a protein preparation that consists of one form of the holoenzyme with more consistent oxidative and electrochemical properties. Validation of theoretical observation was also undertaken by experimental investigation to determine if specific mutations at the T1 Cu site would increase the redox potential of SLAC. Increasing the redox potential in turn increases the half-cell potential, and hence overall productivity, of an enzyme fuel cell.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 17, 2016
- Accession Number
- AD1074925
Entities
People
- Glenn Johnson
- Guinevere Strack
- Heather R. Luckarift
- Karen Farrington
- Lloyd Nadeau
- Matthew Eby
- Plamen Atanassov
- Randi Tatum
- Ruth Pachter
- Sofia Babanova
Organizations
- Air Force Research Laboratory
- Oak Ridge Institute for Science and Education