Validation of Biotechnology for Quantifying the Abundance and Activity of Vinyl-chloride Oxidizers in Contaminated Groundwater

Abstract

The purpose of this guidance document is to provide a detailed technical protocol for conducting Quantitative PCR (qPCR) and Reverse transcription quantitative PCR (RT-qPCR) assays for Vinyl chloride (VC)-oxidizing bacteria in groundwater and sediment samples. Additional guidance on interpreting molecular data in the context of MNA processes and associated statistical analyses will be provided. This guidance document could be useful for site managers interested in utilizing qPCR/ RT-qPCR technology at their site or by a commercial laboratories interesting in transferring the technology for commercial applications.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2018
Accession Number
AD1076497

Entities

People

  • Timothy E Mattes

Organizations

  • University of Iowa

Tags

Communities of Interest

  • Energy and Power Technologies

DTIC Thesaurus Topics

  • Alkenes
  • Bacteria
  • Biotechnology
  • Correlation Analysis
  • Data Science
  • Deoxyribonucleic Acids
  • Ecology
  • Environment
  • Environmental Protection
  • Gene Expression
  • Groundwater
  • Information Science
  • Microorganisms
  • Nucleic Acids
  • Polymerase Chain Reaction
  • Regression Analysis
  • Statistical Analysis

Fields of Study

  • Biology
  • Environmental science

Readers

  • Defense Technology Research and Development.
  • Groundwater Contamination Remediation.
  • Molecular Genetics

Technology Areas

  • Biotechnology
  • Biotechnology - Bioremediation