Folding Complex DNA Nanostructures from Limited Sets of Reusable Sequences
Abstract
Scalable production of DNA nanostructures remains a substantial obstacle to realizing new applications of DNA nanotechnology. Typical DNA nanostructures comprise hundreds of DNA oligonucleotide strands, where each unique strand requires a separate synthesis step. New design methods that reduce the strand count for a given shape while maintaining overall size and complexity would be highly beneficial for efficiently producing DNA nanostructures. Here, we report a method for folding a custom template strand by binding individual staple sequences to multiple locations on the template. We built several nanostructures for well-controlled testing of various design rules, and demonstrate folding of a 6-kb template by as few as 10 unique strand sequences binding to 10 + or - 2 locations on the template strand.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2016
- Accession Number
- AD1077002
Entities
People
- John Garbutt
- Kathy Tsui
- Katy Blumer
- Parsa M. Nafisi
- Shawn M Douglas
- Stefan Niekamp
Organizations
- University of California, San Francisco