Evaluation of the Octet Biolayer Interferometer Platform for Biological Agent Environmental Sampling
Abstract
The U.S. Army Edgewood Chemical Biological Center (now the U.S. Army Combat Capabilities Development Command Chemical Biological Center; Aberdeen Proving Ground, MD) examined the Octet RED384 and HTX instruments (Pall ForteBio; Fremont, CA) to determine the feasibility of using high-throughput, label-free, real-time measurements for analysis of biological molecules by way of biolayer interferometry (BLI). The commercial off-the-shelf Octet platforms were challenged with six bioagents spiked into two environmental sampling matrices, namely, phosphate-buffered saline with 0.1 percent Triton X-100, and dry filter unit extract. Ricinus communis agglutinin, T2 mycotoxin, Bacillus anthracis, NNR-1 spores, Francisella tularensis var. tularensis, Yersinia pestis EV76, and Ebola virus-like particles were selected to determine protein-protein interactions for small (mycotoxin) and large (virus and spore) particles. BLI technology examined limits of detection and true-positive and false-negative responses for the bioagents. Octet biosensors were configured in 96- and 384-well microplates using monoclonal antibodies immobilized to commercially available Dip and Read coupling kits (Pall ForteBio). Antibodies were functionalized to three Octet biosensors that had surface chemistries designed to analyze specific biomolecular interactions: streptavidin, amine reactive second generation, and anti-mouse Fc capture. Overall, the Octet system enabled high-throughput sample analysis with relatively few manipulations and provided a comprehensive data output.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2019
- Accession Number
- AD1077415
Entities
People
- Deborah L. Menking
- Isaac R. Fruchey
- Katherine A. Rhea
- Patricia A. Buckley
Organizations
- United States Army Combat Capabilities Development Command