Totipotent Synthetic Materials
Abstract
This report summarizes the research progress made on a 1-yr seedling project focused on demonstrating proof of concept that stimuli-triggered, cell-free protein synthesis using engineered DNA and machinery from bacterial cells in synthetic compartmentalized materials can provide a path toward a diverse class of materials that can adapt their compositions and properties in ways similar to totipotent plant cells. Our unique approach incorporated stimuli-sensitive riboswitches and cell-free machinery in cell-inspired compartments separated by lipid membranes. The project was successful in demonstrating that multiple kinds of stimuli-responsive riboswitches can function, independently and orthogonally from other stimuli, in the same compartments of the material. Further, our plasmid designs demonstrate the modularity of this approach in creating new types of adaptive, functional materials.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 26, 2019
- Accession Number
- AD1080780
Entities
People
- Donald Leo
- Eric C Freeman
- S. A Sarles
- Scott C. Lenaghan
Organizations
- University of Tennessee