Investigation of Superparamagnetic (Fe3O4) Nanoparticles and Magnetic Field Exposures on CHO-K1 Cell Line
Abstract
Rapid development in nanomaterial synthesis and surface functionalization has led to advanced studies in actuation and manipulation of cellular functions for biomedical applications. One common actuation technique employs externally applied magnetic fields to manipulate magnetic nanomaterials within cells in order to drive or trigger desired effects. While cellular interactions with low-frequency magnetic fields and nanoparticles have been extensively studied, the fundamental mechanisms behind these interactions remain poorly understood. Additionally, modern investigations on these concurrent exposure conditions have been limited in scope, and difficult to reproduce. This study presents an easily reproducible method of investigating the biological impact of concurrent magnetic field and nanoparticle exposure conditions using a well-definedin-vitro CHO-K1 cell line model, with the purpose of establishing grounds for in-depth fundamental studies of the mechanisms driving cellular level interactions. Cells were cultured under various nanoparticle and magnetic field exposure conditions singly or in combination from 0 to 500mu g/ml nanoparticle concentrations and DC, 50 Hz, or 100 Hz magnetic fields with 2.0 mT flux density. Cells were then observed by confocal fluorescence microscopy, and subject to biological assays to determine the effects of concurrent extreme-low frequency magnetic field and nanoparticle exposures on cell-nanoparticle interactions, such as particle uptake and cell viability by MTT assay. Current results indicate little to no variation in effect on cell cultures based on magnetic field parameters alone; however, it is clear that deleterious synergistic effects ofconcurrent exposure conditions exist based on a significant decrease in cell viability when exposed to high concentrations of nanoparticles and concurrent magnetic field.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2016
- Accession Number
- AD1081386
Entities
People
- Bennett L. Ibey
- Larry Estlack
- Saber Hussein
- Tae-youl Choi
- Zachary Coker