High Frequency Application of Nanosecond Pulsed Electric Fields Alters Cellular Membrane Disruption and Fluorescent Dye Uptake

Abstract

Cells exposed to nanosecond-pulsed electric fields (nsPEF) exhibit a wide variety of nonspecific effects, including blebbing, swelling, intracellular calcium bursts, apoptotic and necrotic cell death, formation of nanopores, and depletion of phosphatidylinositol 4,5-biphosphate (PIP2) to induce activation of the inositol trisphosphate/diacylglycerol pathway.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2016
Accession Number
AD1081393

Entities

People

  • Bennett L. Ibey
  • Gleb P. Tolstykh
  • Zachary A Steelman

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Air Force
  • Air Force Research Laboratories
  • Amplitude
  • Biomedical Engineering
  • Confocal Microscopy
  • Electric Fields
  • Fluorescence
  • Frequency
  • Generators
  • Membranes
  • Microscopes
  • Microscopy
  • Nanosecond Time
  • Pulse Generators
  • Radio Frequency
  • Repetition Rate
  • Standards

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Pulsed Power and Plasma Physics.