Molecular Imaging of Human Performance Biomarkers at Cellular Resolution in Vivo
Abstract
Previously we described a novel imaging technique developed in our group, Speckle-Modulating Optical Coherence Tomography (SM-OCT), which dramatically improves the quality of optical coherence tomography images by reducing speckle noise. We have extensively explored the use of SM-OCT for brain imaging, presenting some of the highest resolution images of brain structures taken over a wide field of view of many millimeters. Some of the smaller brain structures are not visible at all in regular OCT images due to speckle noise. In living mice, we find that SM-OCT allows for visualization of distinct cortical layers, as well as individual white matter fascicles. In freshly resected human brain tissue, SM-OCT resolves the first three cortical layers as well as individual myelinated axonal processes, primarily in layers one and three. Finally, our experiments with contrast agents indicate that SM-OCT is highly complementary with our molecular imaging platform MOZART, enabling improved sensitivity of nanoparticle detection. By leveraging these techniques, we demonstrate near real-time tracking of leukocyte migration and distribution within the brain of a living mouse. We believe the resolution and sensitivity of SM-OCT will allow it to be used as a versatile clinical and scientific tool for non-invasive, serial imaging of various neurobiologic processes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2019
- Accession Number
- AD1085617
Entities
People
- Adam De La Zerda
Organizations
- Stanford University