Development of a Novel Segmental Bone Defect Construct

Abstract

This report contains the final summary of all project efforts conducted on the project Development of a Novel Segmental Bone DefectConstruct. The original project hypothesis was that one or more of the SIBLING (small integrin binding, N-linked glycoprotein) proteins isresponsible for recruiting cells for bone tissue repair and regeneration and their use in a tissue engineering scaffold will induce a natural,expedited wound healing response for segmental bone defects. This hypothesis was tested through the completion of four major tasks.Major Task #1 was to determine the SIBLING protein family member that facilitated the best MC3T3-E1 osteoblast-like cell attachment to amultifunctional polyampholyte hydrogel. In Major Task #2, the top performing SIBLING protein was evaluated in primary cell proliferation,differentiation, and penetration into the base case polyampholyte hydrogel. In Major Task #3, a family of polyampholyte hydrogels with arange of mechanical properties was developed. Finally, in Major Task #4 the primary cell proliferation, differentiation, and penetration intopolyampholyte hydrogels with a range of mechanical properties was evaluated.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2019
Accession Number
AD1086081

Entities

People

  • Matthew T. Bernards

Organizations

  • University of Idaho

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Alkenes
  • Biomedical And Dental Materials
  • Buffers (Chemistry)
  • Cell Physiological Processes
  • Chemical Synthesis
  • Chemistry
  • Materials Laboratories
  • Materials Science
  • Materials Testing
  • Organic Chemistry
  • Polymer Chemistry
  • Polymeric Films
  • Proteins
  • Stem Cells

Fields of Study

  • Materials science

Readers

  • Molecular Biology and Genetics
  • Technical Research and Report Writing.
  • Trauma Surgery or Emergency Medicine.