Single-Cell Dissection of Human Pancreatic Islet Dysfunction in Diabetes

Abstract

Islets are composed of > or = 5 endocrine cell types that perform complementary functions to maintain proper glucose homeostasis. This cellular heterogeneity impedes our ability to understand the precise transcriptional repertoire and regulatory landscape of each cell type and to determine how these programs in each cell type are perturbed in type 2 diabetes (T2D). The overarching goal of this project is to determine, with single cell resolution, changes in cellular composition and cell-specific gene expression programs elicited by T2D in human islets using innovative single cell transcriptomic (scRNA-seq; Aim 1) and epigenomic (scATAC-seq; Aim 2) technologies.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2019
Accession Number
AD1087089

Entities

People

  • Michael L Stitzel

Organizations

  • Jackson Laboratory

Tags

DTIC Thesaurus Topics

  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Computational Biology
  • Computational Science
  • Confocal Microscopy
  • Diabetes Mellitus
  • Diseases And Disorders
  • Endocrine Cells
  • Gene Expression
  • Genetic Structures
  • Genetic Variation
  • Genetics
  • Glucose Metabolism Disorders
  • Metabolic Diseases
  • Metabolism

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biology