The Urinary Fungal Mycobiome and Host Responses in Patients with Interstitial Cystitis

Abstract

We have completed recruitment of 200 subjects, have identified distinctive bacterial community types with a relationship to dysbiosis, and developed new techniques for identifying both bacterial and fungal species. We have successfully extracted both fungal and bacterial DNA from the catheterized urine samples and confirmed this through both quantitative polymerase chain reaction (qPCR) and gel electrophoresis. We have refined classification of bacterial communities found in both controls and individuals diagnosed with Interstitial Cystitis and confirmed the connection between specific community types and pelvic pain/disease. We have developed specific probes that allow us to complete fungal analysis. We have developed a more sophisticated approach to biostatistical analysis and are currently completing, proteomics, analysis and Multiple Reaction Monitoring validation.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2019
Accession Number
AD1087630

Entities

People

  • A. L. Ackerman

Organizations

  • Cedars-Sinai Medical Center

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Classification
  • Communities
  • Data Analysis
  • Data Science
  • Diseases And Disorders
  • Genomics
  • Information Science
  • Local Governments
  • Medical Personnel
  • Pain
  • Patent Applications
  • Polymerase Chain Reaction
  • Professional Development
  • Proteomics
  • Statistical Analysis
  • Validation

Fields of Study

  • Biology
  • Environmental science
  • Medicine

Readers

  • Gulf War Illness and Chronic Multisymptom Illness in Veterans.
  • Oncology and Biomarker-Based Cancer Detection.

Technology Areas

  • Biotechnology