Mechanistic and Therapeutic Implications of Spliceosomal Gene Mutations in ER+ Breast Cancer

Abstract

Recent evidence has revealed that altered mRNA splicing is a mechanism by which tumors can derive constitutive, tumor-promoting signals. Recurrent, somatic mutations in the core RNA splicing factor SF3B1 have been found in several malignancies. Through analyses of metastatic breast cancer patients at our center, we have noted hotspot mutations in SF3B1 in up to 6 percent of cases and these are associated with the ER+/HER2- subtype and inferior outcomes in patients. We therefore conducted studies to understand the potential implications of SF3B1 mutations on breast cancer pathogenesis and found that expression of the most common mutant, K700E, led to alterations in RNA splicing and promoted hormone-independent proliferation of ER+ cells. Based on these initial observations, we are now studying the consequences of SF3B1 mutation on breast tumorigenesis, cancer progression, and sensitivity to spliceosomal targeted therapy.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2019
Accession Number
AD1090535

Entities

People

  • Omar Abdel-Wahab
  • Sarat Chandarlapaty

Organizations

  • Sloan-Kettering Institute

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cells
  • Chemotherapy
  • Clinical Trials
  • Contracts
  • Diseases And Disorders
  • Epithelial Cells
  • Gene Expression
  • Hematologic Diseases
  • Inhibitors
  • Leukemia
  • Lymphatic Diseases
  • Mammary Glands
  • Medical Personnel
  • Mutations
  • Neoplasms
  • New York
  • Pathogenesis
  • Peptide Growth Factors
  • Sensitivity
  • Therapy

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Oncology
  • Prostate Cancer Biology.