Acute Pancreatitis as a Model to Predict Transition of Systemic Inflammation to Organ Failure in Trauma and Critical Illness
Abstract
Trauma, extensive burns, bacterial infections, and acute pancreatitis (AP) are common conditions of tissue injury and immune system activation that can result in the systemic inflammatory response syndrome (SIRS). Surprisingly, about half of the patients with SIRS quickly recover, while the others develop a multiorgan dysfunction syndrome (MODS). SIRS and MODS do not occur immediately: SIRS evolves over a 4-12 hour period, while MODS evolves over12-24 hours. Vascular leak syndrome (VLS) is a critical component of the transition from SIRS to MODS. Understanding the mechanism by which SIRS triggers VLS and progresses to MODS is critical to correctly model disease course thereby aiding in treatment of patients. We tested the effect of severe acute pancreatitis patient serum on the viability of human vascular endothelial cells grown in a monolayer and evaluated potential cell death mechanisms. Serum from enrolled subjects was also tested for potential biomarkers in order to better understand course from SIRS to organ failure (OF). Our data shows that main modes of cell death are necrosis and autophagy and that multiple mechanisms are involved in course to OF.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2018
- Accession Number
- AD1093128
Entities
People
- Annette S. Wilson
- David C Whitcomb
Organizations
- University of Pittsburgh