Discovery and Characterization of PRCAT47: A Novel Prostate Lineage and Cancer-Specific Long Noncoding RNA
Abstract
The overall goal of this project is to characterize the functioning mechanism, utility and therapeutic potential of the biomarker PRCAT47 in prostate cancer. During this second period, we investigated the regulatory effect of PRCAT47 on androgen receptor (AR)transcript stability. We also interrogated the role of PRCAT47 in regulating HuR/TIAR-targeted mRNAs. Utilizing a novel RNA in situ hybridization technology optimized within the first period which allows us to assess PRCAT47 expression in prostate tissues, we have not evaluatedPRCAT47 expression in prostate cancer tissue microarrays by RNA-ISH and demonstrate an association with prostate cancer progression. Finally, we also assessed the therapeutic potential of PRCAT47 using cell line models and patient derived organoid cultures.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2019
- Accession Number
- AD1093352
Entities
People
- Rohit Mehra
Organizations
- University of Michigan