Development of a Novel Segmental Bone Defect Construct

Abstract

It is hypothesized that one or more of the SIBLING (small integrin binding, N-linked glycoprotein) proteins is responsible for recruiting cells for bone tissue repair and regeneration and their use in a tissue engineering scaffold will induce a natural, expedited wound healing response for segmental bone defects. To test this hypothesis, four major tasks have been proposed and during this reporting period progress has been made in all four of these areas. Major Task #1 was to evaluate the SIBLING protein family member that facilitated the best MC3T3-E1 osteoblast-like cell attachment to a multifunctional polyampholyte hydrogel. In Major Task #2, the top performing SIBLING protein was to be evaluated in primary cell proliferation, differentiation, and penetration into the base case polyampholyte hydrogel. In addition, in Major Task #4 the primary cell proliferation, differentiation, and penetration into polyampholyte hydrogels with a range of mechanical properties was to be evaluated. The creation of polyampholyte hydrogels with a range of mechanical properties was Major Task #3.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2018
Accession Number
AD1094056

Entities

People

  • Matthew T. Bernards

Organizations

  • University of Idaho

Tags

DTIC Thesaurus Topics

  • Adhesion
  • Alkenes
  • Biomedical Research
  • Bone Marrow
  • Bone Marrow Cells
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Connective Tissue
  • Culture Techniques
  • Engineering
  • Mechanical Properties
  • Physical Properties
  • Polyethylene Glycols
  • Proteins
  • Tissue Engineering

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