Glycocalyx Mechanoregulation of Angiopoietin-2 and Post-Traumatic Lung Injury

Abstract

Preliminary results from our clinical studies show elevated levels of activated heparanase (HPSE) in plasma collected from trauma patientsupon arrival to our Level I Trauma Center. Additionally, these patients have elevated levels of Angiopoeitin-2, a well-established biomarkerof endothelial cell (EC) inflammation. Results from our in vitro studies suggest that degradation of heparan sulfate causes lungmicrovascular EC to produce more Agpt-2, suggestive of a mechanistic linkage between HPSE enzyme activity and dysregulation of Agpt-2levels. Since Agpt-2 increases the permeability of blood vessels and promotes lung injury, blocking the activity of HPSE and preventingAgpt-2 release could be a viable strategy for preventing lung injury in trauma patients. Preliminary data from animal studies show thatblocking HPSE activity with the drug Roneparstat may attenuate pulmonary cytokine production caused by trauma-hemorrhage andresuscitation. These findings are important for the field of trauma research since they address a clinical need for developing therapeuticstrategies that target mechanisms responsible for vascular dysfunction and organ failure in trauma patients.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2019
Accession Number
AD1094152

Entities

People

  • Jillian R Richter

Organizations

  • University of Alabama at Birmingham

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Blood
  • Blood Vessels
  • Cell Membrane Structures
  • Cells
  • Cellular Structures
  • Cytokines
  • Degradation
  • Endothelial Cells
  • Hemorrhage
  • Hemorrhagic Shock
  • Inflammation
  • Maryland
  • Measurement
  • Proteins
  • Resuscitation
  • Wounds And Injuries

Fields of Study

  • Medicine

Readers

  • Immunology and Pathology
  • Trauma Surgery or Emergency Medicine.