Direct Imaging of Modulatory Neurotransmitters Using Synthetic Nanosensors to Understand and Treat Parkinsons Disease: Early Investigator Award

Abstract

Nanosensors for the optical detection of dopamine in brain slice has been validated in wildtype C57/C6 mice as well as a Parkinson's model mouse. Stereotaxic LPS injections into the substantia nigra of mice selectively kills dopaminergic neurons and produces a Parkinson's phenotype. This is confirmed using immunohistochemistry and cell death stains in fixed slice. Using nanosensors, endogenous dopamine release in striatal brain slice is triggered by electrical stimulation and quantified using microscopy. We have established the workflow for this procedure and have prepared a population of Parkinson's and wildtype mice to image in the coming quarter.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2019
Accession Number
AD1094177

Entities

People

  • Jackson T. Del Bonis-o'donnell

Organizations

  • University of California Regents

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Amines
  • Amino Acids
  • Brain
  • California
  • Carbon Nanotubes
  • Cells
  • Chemistry
  • Data Analysis
  • Detection
  • Detectors
  • Diseases And Disorders
  • Governments
  • Image Processing
  • Measurement
  • Medical Personnel
  • Neurons
  • Parkinson'S Disease

Fields of Study

  • Biology

Readers

  • Neurodegenerative Parkinson's Disease and Rickettsial Disease handbook, including the data level of dopamine, BC, neurons, and PD.
  • Neuroscience
  • Oncology and Biomarker-Based Cancer Detection.