Bioresponsive Protein Nanotherapeutics for Lung Cancer Treatment

Abstract

The main objective of this project is to develop a multistage nanoparticle (NP) platform for both effective systemic and cytosolic delivery of therapeutic proteins into tumors, and to explore the potential of this bioresponsive protein nanotherapy in lung cancer treatment. Over the past year, we have made substantial progress and accomplishments under the proposed tasks. We synthesized and characterized a library of pH-responsive polymers. The NP formation by using these polymers was evaluated by TEM and DLS. To improve the intracellular delivery of proteins, we also synthesized tetra-guanidinium saporin conjugates and tested their anti-tumor efficacy in vitro with A549 and H1299 cells. We have further evaluated the pH-responsive polymeric NPs for PTEN protein delivery in terms of cellular uptake by fluorescence imaging and flow cytometry, and PTEN expression by western blot. The pharmacokinetics of the protein NPs was also examined.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2019
Accession Number
AD1094214

Entities

People

  • Jinjun Shi

Organizations

  • Brigham and Women's Hospital

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Cancer
  • Cardiovascular Physiological Processes
  • Department Of Defense
  • Education
  • Engineering
  • Hospitals
  • Information Operations
  • Lung Cancer
  • Maryland
  • Nanotherapy
  • Neoplasms
  • Organic Solvents
  • Professional Development
  • Students
  • Technology Transfer
  • Training

Fields of Study

  • Biology
  • Chemistry

Readers

  • Molecular and Cellular Biochemistry
  • Nanocomposite Materials Science
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech