Exhaled Breath Condensate for Proteomic Biomarker Discovery

Abstract

Exhaled breath condensate (EBC) has been established as a potential source of respiratory biomarkers. Compared to the numerous small molecules identified, the protein content of EBC has remained relatively unstudied due to the methodological and technical difficulties surrounding EBC analysis. In this review, we discuss the proteins identified in EBC, by mass spectrometry, focusing on the significance of those proteins identified. We will also review the limitations surrounding mass spectral EBC protein analysis emphasizing recommendations to enhance BC protein identifications by mass spectrometry. Finally, we will provide insight into the future directions of the EBC proteomics field.

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Document Details

Document Type
Technical Report
Publication Date
Jul 02, 2014
Accession Number
AD1094358

Entities

People

  • Claude C. Grigsby
  • Darrin K. Ott
  • Sean W. Harshman

Organizations

  • Universal Energy Systems

Tags

Communities of Interest

  • Human Systems
  • Space

DTIC Thesaurus Topics

  • Acute Respiratory Distress Syndrome
  • Air Force
  • Air Force Research Laboratories
  • Biological Factors
  • Biological Markers
  • Chemistry
  • Electronic Mail
  • Liquid Chromatography
  • Lung Diseases
  • Mass Spectrometry
  • Molecules
  • Peptides
  • Proteins
  • Proteomics
  • Small Molecules
  • Spectrometry
  • Volatile Organic Compounds

Readers

  • Analytical Chemistry
  • Molecular Genetics
  • Systems Analysis and Design

Technology Areas

  • Biotechnology