Nanoink Printed Amperometric Immunosensor for Rapid and Inexpensive Screening of Tuberculosis
Abstract
The project aims to develop a point-of-care diagnostic platform for tuberculosis diagnosis. The sensor detects Mycobacterium tuberculosis(MTB; H37Ra strain) cells and MPT-64 antigen spiked in human sputum samples. Single walled carbon nanotubes are used as a sensing element for detection of target analytes. In the project period, various SWCNTs-based immunosensors were designed and fabricated. The fabrication and detection protocol was optimized. For specific detection, antibodies to MPT-64 were generated and characterized. Through affinity purification, the antibodies became more sensitive and specific to target analytes. Using the sensor, the detection limit was 106CFU/mL for BCG and 100 ng/mL for MPT-64. In the next project period, the detection protocol will be further optimized to achieve detection limit of 103 CFU/mL (or equivalent concentration to MPT-64).
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2018
- Accession Number
- AD1094509
Entities
People
- Clement Furlong
- Jae-Hyun Chung
- Jong-hoon Kim
Organizations
- University of Washington