MicroRNA in Cerebral Spinal Fluid as Biomarkers of Alzheimer's Disease Risk After Brain Injury
Abstract
A history of TBI increases the odds of developing AD by 2.5 times in the general population, and 4-6times for military veterans. Although significant associations between mild TBI and risk of AD havebeen observed, the precise mechanism by which TBI might lead to AD and/or AD-related symptoms are notyet understood. Histologically, AD is characterized by amyloid- and neurofibrillary protein aggregates,suggesting a loss of protein processing is a key feature of AD. MicroRNAs (miRNA) are small non-codingRNAs that regulate mRNA translation, and may be a significant cause of protein dysregulation.To date, we have established molecular biology techniques that allow us to measure miRNA incerebrospinal fluid (CSF) from living donors. Further, we have established and validated changes in themiRNA using a biostatistical pipeline to identify biomarker candidates from our assays. We are nowbuilding a bioinformatics pipeline to associate altered miRNA signatures with predicted changes in mRNAregulation and altered protein expression that may correlate with AD-related pathologies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2019
- Accession Number
- AD1094636
Entities
People
- Joseph Quinn
- Theresa A. Lusardi
Organizations
- Oregon Health & Science University