Identifying Determinants of PARP Inhibitor Sensitivity in Ovarian Cancer

Abstract

During the course of the grant, we have discovered several novel insights into PARP inhibitor (PARPi) and cisplatin resistance mechanisms. We showed that the BRCA1-delta11q protein is responsible for promoting PARPi resistance in ovarian cancers with exon 11 located BRCA1 mutations. In another aspect of our studies encompassed within this grant, we discovered that BRCA1185delAG alleles were capable of initiating translation at a start site downstream of the mutation-induced stop codon. We also made a BRCA1 null mouse model and subsequently discovered that 53BP1 knockout only partially restores HR in the absence of BRCA1 hypomorphic proteins. In another recent study, our laboratory showed that BRCA2 mutant alleles are capable of expressing proteins that contribute to PARP inhibitor resistance. These studies were published and OC130212 acknowledged in Wang et al., Cancer Research, 2016; Wang et al., Journal of Clinical Investigation, 2016; Nacson et al., Cell Reports, 2018; Park et al., Molecular Cancer Therapeutics, 2019; and Wang et al., Nature Communications, 2019. In terms of career development, I was promoted to associate professor with tenure in May 2019.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2019
Accession Number
AD1094762

Entities

People

  • Neil F. Johnson

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Chromosome Structures
  • Chromosomes
  • Cytoskeleton
  • Gene Expression
  • Genetics
  • Health Services
  • Mass Spectrometry
  • Materials
  • Neoplasms
  • Oncology
  • Proteins
  • Rna Stability
  • Statistical Analysis

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Oncology
  • Research Science/Academic Research