Development of a Supercooled Limb Preservation Protocol

Abstract

The project aims to develop a novel technology to preserve vascular composite allografts for extended periods. This project uses a rat limb transplant model and aims to preserve grafts for 3 days or more. In this first year, we developed a machine perfusion protocol for rat limbs. Several aspects had to be optimized, including media, additives, oxygen carriers among others to identify a protocol that does not lead to ischemia, exhibits sustained low vascular resistance, and energy levels. We further started evaluation of the perfused grafts by transplantation with success in the first tests. We also started optimization of the supercooled graft preservation, which will be the main goal in year 2.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2018
Accession Number
AD1094775

Entities

People

  • Korkut Uygun

Organizations

  • Massachusetts General Hospital

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Albumins
  • Allografts
  • Biomedical Research
  • Composite Materials
  • Department Of Defense
  • Governments
  • Health Services
  • Humanities
  • Local Governments
  • Machines
  • Medical Personnel
  • Patent Applications
  • Polyethylene Glycols
  • Professional Development
  • Students
  • Systems Engineering
  • Transplants

Fields of Study

  • Biology

Readers

  • Trauma Surgery or Emergency Medicine.

Technology Areas

  • Biotechnology