Rational Therapies for Diffuse-Type Gastric Cancer

Abstract

We have collected a panel of 7 gastric cell line models: MKN7, AGS, KATO-III, NCI-SNU-1, NCI-SNU-5, NCI-SNU16 and NCI-N87. Using data available through CCLE and Cosmic Databases (Garnett, 2012 no. 76) (Barretina, 2012 no. 1), we determined the copy number and mutational status of CDH1 and identified a single base pair insertion in AGS cells prematurely terminates CDH1 in exon 12. We have designed and constructed CRISPR reagents to correct this mutation and restore CDH1 function. This isogenic cell line pair will be used to investigate the signaling networks in CDH1-deficient cells compared to CDH1-proficient lines.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2018
Accession Number
AD1094813

Entities

People

  • Eric Collisson

Organizations

  • University of California Regents

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  • Biomedical Research
  • California
  • Cell Line
  • Cells
  • Clinical Trials
  • Department Of Defense
  • Dna Sequence Analysis
  • Drug Therapy
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  • Mutations
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  • Biotechnology