Molecular and Cellular Determinants of Malignant Transformation in Pulmonary Premalignancy

Abstract

During the no-cost extension period we partially completed the Major Task 3. To evaluate the differences in immune landscapes between premalignancy and cancer, the tumor and premalignant microenvironments were assessed for CD3, CD4, CD8, CD11c, CD68, HLA-DR, TIM3, LAG3, Granzyme B, FOXP3, PD-1, PD-L1, Ki67 and cytokeratin expression utilizing multiplex immunofluorescence (MIF) staining. Thus, we significantly expanded the originally proposed immune marker panel, which will allow us to define the infiltrating immune cell landscapes with much higher resolution. The MIF data analysis is ongoing and will be completed utilizing other funding mechanisms.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2019
Accession Number
AD1095175

Entities

People

  • Kostyantyn Krysan

Organizations

  • University of California

Tags

DTIC Thesaurus Topics

  • Adaptive Immunity
  • Algorithms
  • Biological Staining And Labeling
  • Cancer
  • Cells
  • Data Analysis
  • Databases
  • Epithelial Cells
  • Gene Expression
  • Genetic Variation
  • Genetics
  • Identification
  • Institutional Review Board
  • Lung Cancer
  • Lymphocytes
  • Medical Personnel
  • Statistical Analysis

Fields of Study

  • Biology

Readers

  • Immunology
  • Oncology (Cancer Research).
  • Oncology and Biomarker-Based Cancer Detection.