Systems Biology of the Immune Response to Live and Inactivated Dengue Virus Vaccines

Abstract

The objective of this project is to elucidate the immunological mechanisms induced by live-attenuated and purified inactivated dengue virus vaccines administered in a heterologous prime-boost regimen. Innate and adaptive (T and B cell) responses will be measured using molecular and cellular approaches and the data analyzed using a systems biology approach. During the third project year, we performed genomic characterization of study subjects using molecular HLA typing, RNA-seq of unfractionated PBMC, and single-cell RNA-seq of PBMC. Genomic data were analyzed for associations with vaccine regimen and measures of vaccine immunogenicity. Preliminary findings from these analyses are being explored further.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2019
Accession Number
AD1095222

Entities

People

  • Alan L. Rothman
  • Jeffrey R. Currier

Organizations

  • University of Rhode Island

Tags

DTIC Thesaurus Topics

  • B Lymphocytes
  • Biology
  • Biomedical Research
  • Blood
  • Cells
  • Computational Biology
  • Data Analysis
  • Data Sets
  • Gene Expression
  • Genetic Structures
  • Genetics
  • Lymphocytes
  • Rhode Island
  • Systems Biology
  • Universities
  • Vaccination
  • Vaccines

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular and genetic basis of cancer.
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology