Identification of Prostate Cancer Predisposition Genes on the Y Chromosome
Abstract
We hypothesize the existence of Y chromosome genes/variants related to increased risk, and propose multiple genetic analyses to efficiently identify the responsible genes or variants. We used a unique genealogical resource and a novel study design to identify men carrying Y chromosomes exhibiting high- and low-risk of prostate cancer. We sequenced the Y chromosome to identify rare variants associated with risk, and validated candidate variants in additional high- and low-risk Y chromosome carriers. Analysis of the 10 high risk Y sequence data compared to the 10 low risk data identified a set of 3 coding and 3 non-coding candidate variants seen in excess in high-risk Y chromosomes and not observed in the low risk set We selected 100 Utah Y chromosomes for complete Y chromosome sequencing as cases and acquired Y chromosome sequence data for 1,800 control men. Data fur the 100 additional samples was used to statistically test the 6 candidate variants identified in the initial set of 10 high risk cases and 10 controls; there were no significant results. We also used the high-risk YID cases' sequence data to identify 3 additional candidate variants observed in excess in high-risk Y chromosomes; these 3 candidates were tested with a taqman assay in an additional 150 high risk YID samples and 650 low to normal prostate risk YID samples. No significant results were found
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2019
- Accession Number
- AD1095290
Entities
People
- Lisa Cannon-Albright
Organizations
- University of Utah