Autophagosomal Sequestration of Mitochondria as an Indicator of Antiandrogen TherapyResistance of Prostate Cancer (PCa)

Abstract

Purpose: We are investigating if sequestration of metabolically dysfunctional mitochondria by the autophagosomes (mitophagy) imparts anti-androgen resistance and if this phenomenon can be applied in circulating tumor cells in patient blood samples as a biomarker for development of drug resistance. Method: Effects of the anti-androgen enzalutamide on the autophagy and mitophagy of androgen-dependent LNCaP and independent C4-2 and CWR22v1 cells are studied first. Autophagy is monitored by fluorescence of cells with anti-LC3B antibody. Cellular fluorescence due to Mitosox dye oxidation is used to identify mitochondria producing high superoxide (O2-).

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2018
Accession Number
AD1095565

Entities

People

  • George Wilding

Organizations

  • University of Texas at Austin

Tags

DTIC Thesaurus Topics

  • Androgens
  • Antibodies
  • Autophagy
  • Biomedical Research
  • Cell Line
  • Cells
  • Confocal Microscopy
  • Department Of Defense
  • Drug Resistance
  • Energy Transfer
  • High Resolution
  • Medical Personnel
  • Microscopes
  • Microscopy
  • Mitochondria
  • Prostate Cancer
  • Resistance

Fields of Study

  • Biology
  • Medicine

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Molecular and Cellular Biology
  • Prostate Cancer Biology.