Targeting Basal Breast Cancer
Abstract
We hypothesized that Gpr is a highly specific marker of mammary stem cells or early progenitors that become amplified in basal type tumors and that elimination of Gpr+ cells will lead to tumor regression and eradication. To test these hypothese we aimed to: 1) identify, isolate and characterize Gpr+ cells, determine their potency by tracing their lineage, and kill Gpr+ cells and monitor the effect on mammary development; and 2) determine Gpr expression in human breast cancer, and test if ablating Gpr+ cells affects tumorigenesis in murine mammary tumors. In this grant period we have 1) characterized Gpr expression using lac Z reporters, 2) generated Gpr null mice and documented their mammary and eye developmental defects, 3) genetically demonstrated a requirement for the Gpr cytoplasmic and transmembrane domains, 4) begun to track the Gpr lineage by crossing a mouse where the Gpr promoter drives expression of a tamoxifen induced cre recombinase to an inducible R26R-TdTomato reporter line.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2018
- Accession Number
- AD1095849
Entities
People
- Pamela Cowin
Organizations
- Grossman School of Medicine