Strategies to Improve Chronic Nerve Regeneration and Target Reinnervation

Abstract

This study was designed to examine the effects of delivery of GMF-beta to denervated nerve to preserve the reactive state of Schwann cells and delivery of IGF-1 to denervated muscle to preserve muscle bulk and reinnervation capacity using a model of chronic nerve injury. We were able to complete the aim focusing on IGF-1 and demonstrated that IGF-1 encapsulated into slow release nanoparticles is able to preserve muscle bulk during chronic denervation and keeps it ready for reinnervation. We did have some technical issues with the GMF-beta delivery as the commercial sources for GMF-beta were unreliable. Recently we were able to make our own GMF-beta and we are currently evaluating the effects on chronically denervated Schwann cells. As a backup we also developed a non-viral gene delivery approach. We plan to combine these two approaches in the second half of Year 2 to examine the effects on functional recovery after chronic nerve injury.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2018
Accession Number
AD1095909

Entities

People

  • Ahmet Höke
  • Ruifa Mi

Organizations

  • Johns Hopkins University

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Biomolecules
  • Cell Physiological Processes
  • Cells
  • Diameters
  • Electronic Mail
  • Encapsulation
  • Local Governments
  • Materials
  • Nanoparticles
  • Particles
  • Patent Applications
  • Peripheral Nervous System
  • Professional Development
  • Recovery
  • Students
  • Training

Fields of Study

  • Medicine

Readers

  • Marine Propulsion Engineering and Naval Architecture
  • Neurotrauma and Rehabilitation Medicine.
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology