Reinnervation of Paralyzed Muscle by Nerve-Muscle-Endplate Band Grafting

Abstract

This research aimed to assess the efficacy of our recently developed a new surgical technique called nerve-muscle-endplate grafting (NMEG) in the native motor zone (NMZ) for muscle reinnervation in a rat neck muscle model. An NMEG harvested from a donor muscle was implanted into the NMZ of the experimentally denervated muscle. Our experiments showed that NMEG-NMZ technique yielded optimal functional recovery (82% of the control) of the target muscle. Importantly, the outcome of the NMEG-NMZ was further improved by additional use of exogenous neurotrophic factors (ENF) and intraoperative brief electrical stimulation (ES). Specifically, the combination of NMEG-NMZ with ENF and ES resulted in more optimal functional recovery (91% and 90% of the control, respectively) as compared with NMEG-NMZ surgery alone. Our studies have demonstrated that NMEG-NMZ procedure physically facilitates rapid axon-endplate connections and that focal administration of the ENF and intraoperative brief ES result in extensive nerve regeneration and muscle reinnervation.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2018
Accession Number
AD1096238

Entities

People

  • Jingming Chen
  • Liancai Mu
  • Stanislaw Sobotka
  • Themba Nyirenda

Organizations

  • Hackensack University Medical Center

Tags

DTIC Thesaurus Topics

  • Autonomic Nervous System
  • Biomedical Research
  • Cells
  • Health Services
  • Laboratory Animals
  • Parkinson'S Disease
  • Peptide Growth Factors
  • Peripheral Nervous System
  • Skeletal Muscle

Readers

  • Neuroscience
  • Trauma Surgery or Emergency Medicine.