Cell Response Determinants in Laser-Induced Thermal Impacts

Abstract

In contrast to exogenous determinant of cell death (=temperature x time) following thermal stimulation, endogenous determinants turned out to be complexed and highly-regulated involving different proteins and molecules. Hsp70, which had been assumed to be one of the dominant determinants, also seems to be regulated by other different molecules such as VEGF and Thioredoxin, and calcium signaling appeared to be one of the key factors. We have established a real-time temperature measurement method for in-vitro cultures using optoacoustic technique. Using this method, mechanisms of temperature-resolved cell responses and determinants of subsequent cell functions could be investigated more in detail.

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Document Details

Document Type
Technical Report
Publication Date
May 06, 2019
Accession Number
AD1096320

Entities

People

  • Ralf Peter Brinkmann
  • Yoko Miura

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Air Force Research Laboratories
  • Biological Pigments
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Continuous Waves
  • Culture Techniques
  • Cultured Cells
  • Growth Factors
  • Indicator Dyes
  • Laser Applications
  • Laser Beams
  • Lasers
  • Measurement
  • Ophthalmology
  • Proteins
  • Retinal Diseases

Readers

  • Materials Science and Engineering.
  • Molecular and Cellular Biochemistry
  • Oncology (Cancer Research).

Technology Areas

  • Directed Energy