Experiments and Simulations on Short Chain Fatty Acid Production in a Colonic Bacterial Community

Abstract

Understanding how production of specific metabolites by gut microbes is modulated by interactions with surrounding species and by environmental nutrient availability is an important open challenge in microbiome research. As part of this endeavor, this work explores interactions between F. prausnitzii, a major butyrate producer, and B. thetaiotaomicron, an acetate producer, under three different in vitro media conditions in monoculture and coculture. In silico Genome27scale dynamic flux balance analysis (dFBA) models of metabolism in the system using COMETS (Computation of Microbial Ecosystems in Time and Space) are also tested for explanatory, predictive and inferential power.

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Document Details

Document Type
Technical Report
Publication Date
Oct 16, 2018
Accession Number
AD1096837

Entities

People

  • Alla Ostrinskaya
  • Bea Yu
  • Daniel Segrè
  • David Kong
  • Ilija Dukovski
  • Johanna Bobrow
  • Todd Thorsen

Organizations

  • MIT Lincoln Laboratory

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Accuracy
  • Acetic Acid
  • Analysis Of Variance
  • Bacteria
  • Chemistry
  • Culture Techniques
  • Dynamics
  • Electrospray Ionization
  • Engineering
  • Fatty Acids
  • Gut Microbiome
  • Mass Spectrometers
  • Mass Spectrometry
  • Measurement
  • Microbiomes
  • Microorganisms
  • Spectrometers

Fields of Study

  • Biology

Readers

  • Computational Modeling and Simulation
  • Microbial Pathology
  • Ocean-Atmosphere Mesoscale Modeling, Data Assimilation, and Flux Boundary Layers

Technology Areas

  • Biotechnology
  • Space