The Plateau Method for Forensic DNA SNP Mixture Deconvolution

Abstract

Identification of individuals in complex DNA mixtures remains a challenge for forensic analysts. Recent advances in high throughput sequencing (HTS) are enabling analysis of DNA mixtures with expanded panels of Short Tandem Repeats (STRs) and/or Single Nucleotide Polymorphisms (SNPs). We present the plateau method for direct SNP DNA mixture deconvolution into sub-profiles based on differences in contributors DNA concentrations in the mixtures in the absence of matching reference profiles. The Plateau method can detect profiles of individuals whose contribution is as low as 1/200 in a DNA mixture (patent pending).

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Document Details

Document Type
Technical Report
Publication Date
Nov 27, 2017
Accession Number
AD1096904

Entities

People

  • Darrell O Ricke
  • Edward Wack
  • Eric Schwoebel
  • James J Watkins
  • Joe Isaacson
  • Martha Petrovick
  • Philip Fremont-Smith
  • Tara Boettcher

Organizations

  • MIT Lincoln Laboratory

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Air Force
  • Algorithms
  • Amplification
  • Biology
  • Boundaries
  • Buildings And Structures
  • Capillary Electrophoresis
  • Chemistry
  • Chromosomes
  • Computational Biology
  • Data Analysis
  • Detection
  • Dilution
  • Electrophoresis
  • Engineering
  • Genetic Phenomena
  • Genetics
  • Genotypes
  • Identification
  • Law Enforcement
  • Materials
  • Materials Science
  • Plateaus
  • Systems Biology
  • Test Methods

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Seismology
  • Team-Based Human-Centered Cognitive Task Decision Making and Information Performance.