Mechanistic Interpretation of Hypobaria and Hyperoxia Using Omic Technology
Abstract
Sprague-Dawley (SD) rats were exposed to pressures correlating to ambient (CTL; 21 percent O2, 1000 ft) or hyperoxic only (HYP; 100 percent O2, 1000 ft) or high altitude plus hyperoxic (ALT; 100 percent O2, 30,000 ft above sea level) for 6-8 hours. Exposures were repeated 8-10 times over a 2 week period. Proteomic techniques showed that exposure to HYP or ALT for these durations resulted in distinct protein profiles for significantly regulated proteins with trends for pathway regulation in hippocampus and cortex. NMR-based metabolomics indicated exposing rats to hypobaria and/or hyperoxia had no effects on brain lipid metabolite profiles measured in cerebellum or cortex. Hyperoxia did not increase corticosterone in serum while in ALT there was a significant increase compared to control rats. No differences in glutathione were observed in brain or lung tissue between exposure groups and controls. Levels of malondialdehyde (MDA) were significantly higher in cortex of HYP compared to controls. In brainstem for ALT, levels of MDA decreased significantly relative to control and differed significantly between AL and HYP. Electrophysiology suggested repeated prolonged exposures to 100 percent oxygen results in hyper-excitability of hippocampal neurons. No changes were seen by histopathological examination of brains from exposed rats.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2020
- Accession Number
- AD1097192
Entities
People
- Andy Neufelt
- April Daubenspeck
- Cary Honnold
- Correy R. Vigil
- David R. Mattie
- Deirdre A. Mahle
- Joyce G. Rohan
- Latha Narayanan
- Matthew W. Grogg
- Meghan K. Makley
- Michael C. Moulton
- Molly E. Chapleau
- Nicholas V. Reo
- Sarah T. Pfahler
Organizations
- Henry M. Jackson Foundation for the Advancement of Military Medicine