Nanoink Printed Amperometric Immunosensor for Rapid and Inexpensive Screening of Tuberculosis

Abstract

The project aimed to develop a point-of-care diagnostic platform for tuberculosis diagnosis. The sensor detected Mycobacterium tuberculosis (MTB; H37Ra strain) cells and MPT-64 antigen spiked in samples from tongue swabs and sputa. The fabrication protocol using single walled carbon nanotubes were developed for an immuno-resistive sensor to detect target analytes. We achieved and exceeded the original project goals using the developed sensing platform. For sputum samples, the magnetic enrichment of targets was combined with the SWCNT sensors. The detection limits for MTB and MPT64 spiked in sputa were 100 CFU/mL and 1ng/mL, respectively. The detection limits were 10 CFU/mL for MTB and 100 ng/mL of MPT64 in tongue swab samples with the detection time of 30 minutes. The detection limit was comparable to PCR without requiring bacteriological culture, centrifugation, or nucleic acid amplification.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2020
Accession Number
AD1097371

Entities

People

  • Clement Furlong
  • Jae-Hyun Chung
  • Jong-hoon Kim

Organizations

  • University of Washington

Tags

Communities of Interest

  • Advanced Electronics
  • Sensors

DTIC Thesaurus Topics

  • Biosensors
  • Carbon Nanotubes
  • Chemistry
  • Detection
  • Detectors
  • Fabrication
  • Health Services
  • Manufacturing
  • Materials Laboratories
  • Materials Processing
  • Materials Science
  • Materials Testing
  • Medical Personnel
  • Nanotechnology
  • Point-Of-Care Diagnostic Testing
  • Two Dimensional
  • Wearable Technology

Readers

  • Medical Imaging.
  • Microbial Pathology
  • Nanocomposite Materials Science

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech