Ultra High-Throughput Drug Screen for Lipid-Regulated Ion Channels

Abstract

A dearth of truly high throughput mechanisms to screen ion channels has hampered the development of pharmacological tools and therapeutics for effectively treating diseases involving ion channels (channelopathies). Ion channels are drug targets for pain, cardiac function, and neurological disorders. We have developed a soluble assay for ion channels using a phosphatidylinositol 4,5-bisphosphate fluorescentprobe (FL-PIP2) and detergent purified two pore domain potassium (K2P) and inward rectifying potassium (Kir) channels fused with a nano luciferece luminescent protein (n-Luc). The assay works by bioluminescence energy transfer (BRET).

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2019
Accession Number
AD1099404

Entities

People

  • Scott B. Hansen

Organizations

  • Scripps Research

Tags

DTIC Thesaurus Topics

  • Bioluminescence
  • Biomedical Research
  • Cells
  • Chemical Compounds
  • Department Of Defense
  • Diseases And Disorders
  • Energy Transfer
  • Fish
  • Luminescent Proteins
  • Medical Personnel
  • Molecules
  • Pain
  • Potassium
  • Proteins
  • Small Molecules
  • Students
  • Throughput

Fields of Study

  • Biology
  • Chemistry

Readers

  • Molecular Genetics
  • Neuroscience
  • Plasma Physics.