Effects of Differential Glycosylation on Filovirus Glycoprotein Immunogenicity
Abstract
All current filovirus vaccine platforms utilize the highly glycosylated filovirus attachment protein (glycoprotein, or GP1,2) as an antigen. However, the different vaccine platforms are expressed in various cell types that endow differential glycosylation on GP1,2s, and the importance of the various types of glycosylation in promoting immunogenicity of filovirus GPs is not well known. In this project, we: 1) determined N- and O-linked glycan patterns on filovirus GP1,2 expressed in mammalian, insect, and plant cells; 2) determined that insect cell-generated glycosylated GP1,2s had increased in vitro immunogenicity in dendritic cells; and 3) found that glycosylation of GP1,2 affected in vitro antibody and T cell responses.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2020
- Accession Number
- AD1103070
Entities
People
- Amanda L. Collar
- C. Ye
- Elizabeth C. Clark
- Steven B. Bradfute
Organizations
- University of New Mexico