Instrumentation for Single Molecule Fluorescence Lifetime Imaging in Living Systems
Abstract
Developed was a confocal attachment, that incorporated a fast piezo scanner, a high NA microscope objective, and a picosecond super continuum laser that was interfaced with an existing Guide Star microscope and TTTR-TCSPC instrumentation to create a fluorescence lifetime imaging (FLIM) capability with single molecule sensitivity along with its current state-of-the-art super-resolution capability. This instrument was calibrated to account for the absolute number of photons emitted from an individual probe molecule; giving rise to the real-time quantum calibration of probes in situ.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 12, 2019
- Accession Number
- AD1103122
Entities
People
- James A Brozik
Organizations
- Washington State University