CARS spectroscopy of Aspergillus nidulans spores

Abstract

Coherent Anti-Stokes Raman Spectroscopy (CARS) is performed on single spores (conidia) of the fungus Aspergillus nidulans in order to establish a baseline measurement for fungal spores. Chemical maps of single spores are generated and spectral differentiation between the cell wall and the cytoplasm is achieved. Principal Component Analysis of the measured spectra is then completed as a means to quantify spore heterogeneity. Applications range from the quick and accurate diagnosis of public health concerns to real-time agricultural and environmental sensing of fungal symbionts and pathogens.

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Document Details

Document Type
Technical Report
Publication Date
Feb 11, 2019
Accession Number
AD1104117

Entities

People

  • Alexei Sokolov
  • Benjamin D. Strycker
  • Blake Commer
  • Brian D. Shaw
  • Marlan Scully
  • Zehua Han

Organizations

  • Texas A&M University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Cells
  • Cellular Structures
  • Complex Mixtures
  • Cytoplasm
  • Fungi
  • Health
  • Identification
  • Lasers
  • Materials
  • Measurement
  • Photonic Crystals
  • Public Health
  • Raman Spectra
  • Raman Spectroscopy
  • Spectra
  • Spectroscopy
  • Waveplates

Readers

  • Microbial Pathology
  • Military/Explosive Ordnance Disposal (EOD) Technology
  • Optical Physics and Photonics.