The Urinary Fungal Mycobiome and Host Responses in Patients with Interstitial Cystitis
Abstract
The overall hypothesis of the original project was that uncultivated commensal microbial communities and their interactions with the host are associated with the development of IC/BPS and that the resultant inflammatory host responses, assessed in the urine and blood, create a diagnostic signature specific to IC/BPS. We used two complementary approaches to test this hypothesis: (1) state-of-the-art next-generation sequencing (NGS) resources in microbial community profiling to define fungal and bacterial microbiota of IC/BPS patients in comparison to normal subjects, and (2) proteomic analysis of urine and blood from IC/BPS subjects to identify a specific signature correlating with disease phenotype and severity.We have made significant progress in each of our aims, identifying clinically relevant microbial biomarkers that 1) correlate with IC/BPS, 2) distinguish bladder pain from alternate patterns of pelvic pain refractory to interstitial cystitis treatments and 3) associate with specific inflammatory profiles. We have identified symptom-specific urinary microbial profiles, urotypes, associated with bladder and pelvic pain that are useful as diagnostic biomarkers. In addition, proteomic profiling of IC/BPS subgroups revealed distinct inflammatory associations. These results indicate promising pathways for further research into the mechanisms behind IC/BPS and potential treatment modalities.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2020
- Accession Number
- AD1104270
Entities
People
- A. L. Ackerman
Organizations
- Cedars-Sinai Medical Center